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2 edition of Rapid, sensitive methods for enumeration and enterotoxin assay for Staphylococcus aureus found in the catalog.

Rapid, sensitive methods for enumeration and enterotoxin assay for Staphylococcus aureus

Lee Russon Chugg

Rapid, sensitive methods for enumeration and enterotoxin assay for Staphylococcus aureus

by Lee Russon Chugg

  • 307 Want to read
  • 18 Currently reading

Published .
Written in English

    Subjects:
  • Staphylococcus.

  • Edition Notes

    Statementby Lee Russon Chugg.
    The Physical Object
    Pagination[10], 82 leaves, bound :
    Number of Pages82
    ID Numbers
    Open LibraryOL14239493M

    (Staphylococcus aureus), are a majorbacterial contaminant water has included a method for the enumeration of total staphylococci or S. aureus in swimming pool water, these The method is rapid, technically simple, and inexpensive and yields easily read results. These qualities. Application Staphylococcal enterotoxin B (SEB) which binds to MHC class II molecules and specific Vβ regions of T cell receptors (TCR), resulting in the activation of both monocytes/macrophages and T lymphocytes may be used as a superantigen and immune system activator. SEB may be used as a reference material in the development of SEB detection systems.

    The 3M Petrifilm Staph Express Count plate method was compared with AOAC Official Method for the enumeration of Staphylococcus aureus in selected foods. 11 Reali D () Enterotoxin A and B production in strains of Staphylococcus aureus isolated from human beings and foods.]. Hyg. Camb. 88, 12 Dornbusch K, Hallander H 0 and Lofquist F () Extra­ chromosomal control of methicillin resistance and toxin production in Staphylococcus aureus.]. Bacteriol. 98, ­

    Keywords: Staphylococcus aureus, PCR assay, enterotoxin, food poisoning, SEA, SEB, SEC, SED, SEE Introduction With the rapid increase in the out breaks of food poisoning Surveillance of food borne diseases Staphylococcus aureus is one of the most common clinical and food borne pathogen. It is. The latex agglutination test is used as a rapid screening method, while the tube coagulase test is generally accepted as the “gold standard” for identi-fication of S. aureus (Wilkerson et al., ). Although the tube coagulase test has been recognized as a principal tool in the identification of S. aureus, the test takes about 24 h to.


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Rapid, sensitive methods for enumeration and enterotoxin assay for Staphylococcus aureus by Lee Russon Chugg Download PDF EPUB FB2

INTRODUCTION. Staphylococcus aureus Food Poisoning (FP) is a common cause of food-borne disease worldwide ().Classically, enterotoxins from Staphylococcus aureus strains can be classified into 18 serological types: A-U (except S, F and T) ().Most enterotoxin serotypes are heat stable and may resist inactivation by gastrointestinal proteases like by: The test requires 3 h to complete and yields a purple stain at the site of enterotoxin B-producing colonies, thus allowing direct enumeration of confirmed S.

aureus in foods within 27 h. The method should be applicable to other enterotoxins of S. by: Staphylococcus aureus is a major human pathogen that produces a wide variety of exoproteins that cause various types of disease symptoms.

Some strains produce pyrogenic exotoxins, such as staphylococcal enterotoxins (SEs) and toxic shock syndrome toxin 1 (TSST-1) ().Today, up to 15 SEs are known; the last one discovered was the recently identified SE U (SEU) ().Cited by: Rapid Method for Detection of Staphylococcus aureus Enterotoxins in Food develo p a rapid method to test The detection of uspA gene is considered to be a highly sensitive and specific.

Staphylococcus aureus is an important human pathogen that not only causes skin and respiratory infections, but also induces staphylococcal food poisoning through its production of enterotoxins and other superantigens. The ability of S. aureus superantigens such as enterotoxins to bind directly to major histocompatibility complex (MHC) class II molecules on antigen-presenting cells Cited by: 1.

Rapid, sensitive methods for enumeration and enterotoxin assay for Staphylococcus aureus. Abstract. Graduation date: A selective, differential plating medium for the enumeration of\ud coagulase-positive staphylococci was developed.

The ingredients of\ud this medium were analysed for their effect on normal, heat treated\ud and frozen. aureus.

Methods used to detect and enumerate S. aureus depend on the reasons for testing the food and on the past history of the test material. Processed foods may contain relatively small numbers. RAPID, SENSITIVE METHODS FOR ENUMERATION AND ENTEROTOXIN ASSAY FOR STAPHYLOCOCCUS AUREUS INTRODUCTION The large number of selective and diagnostic media which have been developed for the isolation and enumeration of staphylococci indi-cates that the ideal medium has not yet been developed.

Food samples. Rapid S. aureus identification methods Staphylococcus spp., other than S. aureus, as well as other bacteria can form typical black colonies with or without halos on BP agar.

Such colonies must be tested for coagulase to identify the S. aureus; comm.'cially available kits can provide a rapid result in detecting the staphylococcal protein A as. Vandenbosch LL, Fung DY, Widomski M. Optimum temperature for enterotoxin production by Staphylococcus aureus S-6 and in liquid medium.

Appl Microbiol. Mar; 25 (3)– [PMC free article] Weirether FJ, Lewis EE, Rosenwald AJ, Lincoln RE. Rapid quantitative serological assay of staphylococcal enterotoxin B. Appl Microbiol. Silverman SJ, Knott AR, Howard M,Rapid, sensitive assay for staphylococcal enterotoxin and a comparison of serological methods, Appl Microbiol., – Google Scholar Staphylococcal enterotoxin B, from Staphylococcus aureus (S.

aureus), is one of the most potent bacterial superantigens with profound toxic effects on the immune system. It is associated with food poisoning, toxic shock, atopic dermatitis, asthma, and nasal polyps in humans.

The current diagnostic methods for staphylococcal enterotoxin are mainly based on traditional monoclonal antibodies.

The LAMP assay was very specific for Staph. aureus, and the presence of other contaminating bacterial DNAs and food matrix did not interfere or inhibit the LAMP assay. Conclusions: The template DNA extraction method developed in this study for food samples is simple, rapid and cost‐effective.

LAMP was found to be less sensitive to matrix. Abstract. A rapid and sensitive screening sandwich enzyme-linked immunosorbent assay (ELISA) was developed for the detection of staphylococcal enterotoxin B (SEB) in cheese by using a highly avid anti-SEB antibody (Ab) as the capture Ab (CAb) and as the biotinylated Ab conjugate.

The trend in S. aureus enterotoxin detection and quantification is enhanced by the desire to reduce the time from sampling to identification and confirmation in the laboratory. These commercially available rapid methods (e.g., classical enzyme-linked immunosorbent assay (ELISA)) can detect approximately nanogram of toxin/g of food.

The culture supernatant was from a fermentation of methicillin-sensitive derivative of Staphylococcus aureus A (Johansson et al., ). The culture supernatant was received after centrifugation, sterile filtration, and adsorption of the protein.

The supernatant obtained had a staphylococcal enterotoxin B (SEB) content of mg/dm 3. Aims: The study describes the development of simple and rapid DNA extraction method in combination with loop‐mediated isothermal amplification (LAMP) to detect enterotoxigenic Staphylococcus aureus in food samples.

Methods and Results: In this study, isolation of genomic DNA of enterotoxigenic Staph. aureus from spiked milk, milk burfi, khoa, sugarcane juice and boiled rice.

Staphylococcus aureus (S. aureus) is of great importance and is a leading cause of food poisoning, which is a public health concern in terms of the frequency and seriousness of the disease.

In the present study, RPA and real-time RPA assays were developed and validated to detect S. aureus with high sensitivity and specificity by targeting the nuc gene for the first time.

The availability of highly productive and selective media for the detection and enumeration of Staphylococcus aureus is of great importance in routine food surveillance programmes. In this chapter recent developments in selective and diagnostic media, especially for stressed S.

aureus cells and for food samples with high amounts of accompanying flora, are reviewed. Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA), is a major bacterial pathogen associated with nosocomial and community-acquired S.

aureus infections all over the world. A rapid detection assay for staphylococcal gene of nuc and mecA is needed. In this study, a rapid identification assay based on the loop-mediated isothermal amplification (LAMP) method was.

isolation and enumeration of S. aureus were performed using the Petrifilm TM-Rapid S. aureus Plate Count method. The presence of enterotoxin in the cheese samples was analyzed by the Optimal Sensitivity Plate (OSP) method and the ELFA-VIDAS®-Staph enterotoxin-II assay.

S. aureus strains were tested for their ability to produce enterotoxins.The aim of this study was to describe the microbiological characteristics and profile of genes encoding enterotoxins in 95 Staphylococcus aureus isolates obtained between April and December.This method was able to accurately quantify S.

aureus from milk sample with a relative accuracy of to %. The novel assay successfully detected the presence of as low as 50 copies/mL or copies/g of S. aureus in real food matrices. Introduction Staphylococcus aureus is an opportunistic pathogen and a major causal agent of food poisoning.